grID Information

Summary

grID: hs025713227
Sequence (23-mer): AGGAGGAGCAGAGAGCGAAGCGG
grID Score: high (700)
Doench (2014) Score: 0.41078
Doench (2016) Score: 0.63076
Cas9: S. pyogenes

Genomic Context

Genomic Location: chr12:6643698-6643676
Strand: -
gRNA location: exon:refGene(NM_002046.exon0)

Nearest Genes

refseqgenechromstartenddistance
NM_002046 GAPDHchr12664358466475370
NM_001256799 GAPDHchr1266444086647537732
NM_014865 NCAPD2chr12660329766411322566
NM_001039670 IFFO1chr12664869366652495017
NM_001193457 IFFO1chr12664869366652495017

DNase I hypersensitive sites from ENCODE

DNase I hypersensitivity region:chr12:6643560-6643710
Numbers of Cell Types:123
Cell Types:8988t, A549, AG04449, AG04450, AG09309, AG09319, AG10803, Adult_CD4_Th0, AoAF, AoSMC, BE2_C, BJ, Caco-2, CD14+, CD20+, CD34+_Mobilized, CMK, Chorion, Cll, HSMM_emb, Fibrobl, Fibrop, GM06990, GM12864, GM12865, GM12878, Gliobla, Gm12891, Gm12892, Gm18507, Gm19238, Gm19239, Gm19240, H9es, HAEpiC, HAc, HA-h, HBMEC, HCF, HCFaa, HCM, HCPEpiC, HCT-116, HConF, HEEpiC, H1-hESC, HFF, HFF-Myc, HGF, HIPEpiC, HL-60, HMEC, HMF, HMVEC-LBl, HMVEC-LLy, HMVEC-dAd, HMVEC-dBl-Ad, HMVEC-dBl-Neo, HMVEC-dLy-Ad, HMVEC-dLy-Neo, HMVEC-dNeo, HNPCEpiC, HPAEC, HPAF, HPF, HPdLF, HRCEpiC, HRE, HRGEC, HRPEpiC, HSMM, HSMM_FSHD, HUVEC, HVMF, HeLa-S3, HeLa-S3s3Ifna4h, HepG2, Hepatocytes, HPDE6-E6E7, HTR8svn, Huh-7, Huh-7.5, Ips, Ishikawa, Ishikawa, Jurkat, K562, LNCap, LNCaP, MCF-7, MCF-7, Medullo, Melano, Myometr, NB4, NH-A, NHDF-Ad, NHDF-neo, NHEK, NHLF, NT2-D1, Osteobl, PanIsletD, Panislets, Phte, PrEC, Progfib, RPTEC, RWPE1, SAEC, SK-N-SH, SK-N-MC, SkMC, Stellate, T-47D, Urothelia, UrotheliaUt189, WERI-Rb-1, WI-38, WI-38_TAM, H1-hESC, Th1, Th2,

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Sequence Analysis

Target Sequence (RNA): AGGAGGAGCAGAGAGCGAAG
Target Sequence Occurrence: unique
PAM motif: CGG
Homopolymeric Runs: none
Pol III Terminator: none

Thermodynamic Properties

GC-content (%): 60
GC-score: moderate
Tm (°C): 67.08
Tm score: moderate
RNAfold (dot bracket): .......((.....))....
deltaG (Kcal/mol): -0.50
Predicted Folding: very low secondary structure

Offtarget Analysis

Total Predicted Genomic Targets: 43
Total Genomic Sites With 0 Mismatches: 1
Total Genomic Sites With 1 Mismatches: 1
Total Genomic Sites With 2 Mismatches: 4
Total Genomic Sites With 3 Mismatches: 37
1chr12:6643675-66436980
CCGCTTCGCTCTCTGCTCCTCCT
|||||||||||||||||||||||
CCGCTTCGCTCTCTGCTCCTCCT
2chr8:144272921-1442729441
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*||||||||||||||
AGGAGGAGGAGAGAGCGAAGCGG
3chr16:1630460-16304832
CCGCTTCGCTCTCTGCTCCTCCT
||||||||||||||**|||||||
CCGCTTCGCTCTCTCGTCCTCCT
4chrX:49524180-495242032
AGGAGGAGCAGAGAGCGAAGCGG
|||||||*||||||||||||*||
AGGAGGAACAGAGAGCGAAGGGG
5chr3:11174761-111747842
AGGAGGAGCAGAGAGCGAAGCGG
*||||||||||||||*|||||||
TGGAGGAGCAGAGAGTGAAGCGG
6chr10:6078400-60784232
CCGCTTCGCTCTCTGCTCCTCCT
||||*||*|||||||||||||||
CCGCATCCCTCTCTGCTCCTCCT
7chr12:13016229-130162523
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||||||*||
AGGAGGAAGAGAGAGCGAAGGGG
8chr3:186910284-1869103073
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||||||*||
AGGAGGAAGAGAGAGCGAAGGGG
9chr14:83036372-830363953
CCGCTTCGCTCTCTGCTCCTCCT
||||||*|||||||**|||||||
CCGCTTGGCTCTCTCGTCCTCCT
10chr1:6784292-67843153
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||||||*||
AGGAGGAAAAGAGAGCGAAGGGG
11chr6:137919577-1379196003
CCGCTTCGCTCTCTGCTCCTCCT
||*|||||||||||**|||||||
CCCCTTCGCTCTCTCGTCCTCCT
12chr15:45326884-453269073
CCGCTTCGCTCTCTGCTCCTCCT
||*||||||||||*||*||||||
CCCCTTCGCTCTCAGCACCTCCT
13chr5:16055461-160554843
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||||||*||
AGGAGGACGAGAGAGCGAAGTGG
14chr3:19188578-191886013
CCGCTTCGCTCTCTGCTCCTCCT
|||||||*||||||*||||*|||
CCGCTTCCCTCTCTCCTCCACCT
15chr12:132868776-1328687993
CCGCTTCGCTCTCTGCTCCTCCT
|||||*|||||||||||*|*|||
CCGCTACGCTCTCTGCTGCACCT
16chr5:122976928-1229769513
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||*||||||
AGGAGGAAGAGAGAGCAAAGCGG
17chr5:177988981-1779890043
AGGAGGAGCAGAGAGCGAAGCGG
|*||||*||||||||||||||*|
AAGAGGCGCAGAGAGCGAAGCAG
18chr4:126512857-1265128803
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|*||||||||||||
AGGAGGAAGACAGAGCGAAGCGG
19chr7:104156642-1041566653
AGGAGGAGCAGAGAGCGAAGCGG
|||||*||*|*||||||||||||
AGGAGAAGTACAGAGCGAAGCGG
20chr9:976281-9763043
AGGAGGAGCAGAGAGCGAAGCGG
*|||||||*||||||*|||||||
GGGAGGAGAAGAGAGAGAAGCGG
21chr1:43471918-434719413
CCGCTTCGCTCTCTGCTCCTCCT
||*|||*||||||||||||*|||
CCCCTTGGCTCTCTGCTCCACCT
22chr6:160390332-1603903553
CCGCTTCGCTCTCTGCTCCTCCT
||||**||||||||||||||*||
CCGCAACGCTCTCTGCTCCTGCT
23chr13:77396114-773961373
CCGCTTCGCTCTCTGCTCCTCCT
||*||||*||||||||||||*||
CCCCTTCCCTCTCTGCTCCTGCT
24chr4:17424831-174248543
AGGAGGAGCAGAGAGCGAAGCGG
|||||||*||||||||*|||*||
AGGAGGAACAGAGAGCAAAGGGG
25chr6:35369238-353692613
CCGCTTCGCTCTCTGCTCCTCCT
||*|||*|||||||||||||||*
CCCCTTGGCTCTCTGCTCCTCCA
26chr10:133184897-1331849203
AGGAGGAGCAGAGAGCGAAGCGG
*||||||||||||*|*|||||||
GGGAGGAGCAGAGGGAGAAGCGG
27chr3:14302029-143020523
AGGAGGAGCAGAGAGCGAAGCGG
||*||||||||||||*||||*||
AGCAGGAGCAGAGAGAGAAGGGG
28chrX:38284962-382849853
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*|||||||*|||*||
AGGAGGAGTAGAGAGCTAAGAGG
29chr11:59965353-599653763
CCGCTTCGCTCTCTGCTCCTCCT
||*||||*||||||*||||||||
CCCCTTCCCTCTCTCCTCCTCCT
30chr11:73715410-737154333
CCGCTTCGCTCTCTGCTCCTCCT
|*|||||*||||||||||||*||
CGGCTTCCCTCTCTGCTCCTGCT
31chr10:93438057-934380803
AGGAGGAGCAGAGAGCGAAGCGG
|||*|||||||||||*|||||*|
AGGGGGAGCAGAGAGAGAAGCAG
32chr19:47572388-475724113
CCGCTTCGCTCTCTGCTCCTCCT
||*|*|||||||||*||||||||
CCCCATCGCTCTCTCCTCCTCCT
33chr1:32928769-329287923
CCGCTTCGCTCTCTGCTCCTCCT
||*||||*||||||*||||||||
CCCCTTCCCTCTCTCCTCCTCCT
34chr15:41164702-411647253
CCGCTTCGCTCTCTGCTCCTCCT
||*||||*||||||*||||||||
CCCCTTCCCTCTCTCCTCCTCCT
35chr10:22061873-220618963
AGGAGGAGCAGAGAGCGAAGCGG
|||||||*||||||||||*|*||
AGGAGGAACAGAGAGCGAGGGGG
36chr20:12613999-126140223
CCGCTTCGCTCTCTGCTCCTCCT
||*||||*||||||*||||||||
CCCCTTCCCTCTCTCCTCCTCCT
37chr19:8018199-80182223
CCGCTTCGCTCTCTGCTCCTCCT
||*||||*||||||*||||||||
CCCCTTCCCTCTCTCCTCCTCCT
38chr10:133184444-1331844673
AGGAGGAGCAGAGAGCGAAGCGG
|||||||||||||||*||*||*|
AGGAGGAGCAGAGAGAGAGGCAG
39chr1:158029953-1580299763
CCGCTTCGCTCTCTGCTCCTCCT
||*|||**|||||||||||||||
CCCCTTGCCTCTCTGCTCCTCCT
40chr11:24966728-249667513
CCGCTTCGCTCTCTGCTCCTCCT
||||*||**||||||||||||||
CCGCATCCGTCTCTGCTCCTCCT
41chr10:133183666-1331836893
AGGAGGAGCAGAGAGCGAAGCGG
|||||||||||||||**||||*|
AGGAGGAGCAGAGAGAAAAGCAG
42chr16:90026480-900265033
AGGAGGAGCAGAGAGCGAAGCGG
||||||||||||||||*|*|*||
AGGAGGAGCAGAGAGCCAGGTGG
43chr10:32430180-324302033
AGGAGGAGCAGAGAGCGAAGCGG
||||||||||||||*|*|||*||
AGGAGGAGCAGAGATCTAAGAGG
1
Number of Mismatches:0
Location:chr12:6643675-6643698
Genomic Sequence:AGGAGGAGCAGAGAGCGAAGCGG
Strand:-
Genomic Region:exon(NM_002046.exon0)
3' PAM Orientation:AGGAGGAGCAGAGAGCGAAGCGG
Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||||||||||||||||||
AGGAGGAGCAGAGAGCGAAGCGG
Mismatch: 


2


Number of Mismatches:1


Location:chr8:144272921-144272944


Genomic Sequence:AGGAGGAGGAGAGAGCGAAGCGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAGGAGAGAGCGAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*||||||||||||||
AGGAGGAGGAGAGAGCGAAGCGG


Mismatch:

........G..............
Distal seed mismatch


3


Number of Mismatches:2


Location:chr16:1630460-1630483


Genomic Sequence:AGGAGGACGAGAGAGCGAAGCGG


Strand:-


Genomic Region:gene(IFT140)



3' PAM Orientation:AGGAGGACGAGAGAGCGAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**||||||||||||||
AGGAGGACGAGAGAGCGAAGCGG


Mismatch:

.......C...............
........G..............
Distal seed mismatch
Distal seed mismatch


4


Number of Mismatches:2


Location:chrX:49524180-49524203


Genomic Sequence:AGGAGGAACAGAGAGCGAAGGGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAACAGAGAGCGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||*||||||||||||*||
AGGAGGAACAGAGAGCGAAGGGG


Mismatch:

.......A...............
....................G..
Distal seed mismatch
PAM N Offtarget


5


Number of Mismatches:2


Location:chr3:11174761-11174784


Genomic Sequence:TGGAGGAGCAGAGAGTGAAGCGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:TGGAGGAGCAGAGAGTGAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
*||||||||||||||*|||||||
TGGAGGAGCAGAGAGTGAAGCGG


Mismatch:

T......................
...............T.......
Distal mismatch
Proximal seed mismatch


6


Number of Mismatches:2


Location:chr10:6078400-6078423


Genomic Sequence:AGGAGGAGCAGAGAGGGATGCGG


Strand:-


Genomic Region:gene(IL2RA)



3' PAM Orientation:AGGAGGAGCAGAGAGGGATGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||||||||||*||*||||
AGGAGGAGCAGAGAGGGATGCGG


Mismatch:

...............G.......
..................T....
Proximal seed mismatch
Proximal seed mismatch


7


Number of Mismatches:3


Location:chr12:13016229-13016252


Genomic Sequence:AGGAGGAAGAGAGAGCGAAGGGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAAGAGAGAGCGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||||||*||
AGGAGGAAGAGAGAGCGAAGGGG


Mismatch:

.......A...............
........G..............
....................G..
Distal seed mismatch
Distal seed mismatch
PAM N Offtarget


8


Number of Mismatches:3


Location:chr3:186910284-186910307


Genomic Sequence:AGGAGGAAGAGAGAGCGAAGGGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAAGAGAGAGCGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||||||*||
AGGAGGAAGAGAGAGCGAAGGGG


Mismatch:

.......A...............
........G..............
....................G..
Distal seed mismatch
Distal seed mismatch
PAM N Offtarget


9


Number of Mismatches:3


Location:chr14:83036372-83036395


Genomic Sequence:AGGAGGACGAGAGAGCCAAGCGG


Strand:-


Genomic Region:intergenic



3' PAM Orientation:AGGAGGACGAGAGAGCCAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||*||||||
AGGAGGACGAGAGAGCCAAGCGG


Mismatch:

.......C...............
........G..............
................C......
Distal seed mismatch
Distal seed mismatch
Proximal seed mismatch


10


Number of Mismatches:3


Location:chr1:6784292-6784315


Genomic Sequence:AGGAGGAAAAGAGAGCGAAGGGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAAAAGAGAGCGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||||||*||
AGGAGGAAAAGAGAGCGAAGGGG


Mismatch:

.......A...............
........A..............
....................G..
Distal seed mismatch
Distal seed mismatch
PAM N Offtarget


11


Number of Mismatches:3


Location:chr6:137919577-137919600


Genomic Sequence:AGGAGGACGAGAGAGCGAAGGGG


Strand:-


Genomic Region:intergenic



3' PAM Orientation:AGGAGGACGAGAGAGCGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||||||*||
AGGAGGACGAGAGAGCGAAGGGG


Mismatch:

.......C...............
........G..............
....................G..
Distal seed mismatch
Distal seed mismatch
PAM N Offtarget


12


Number of Mismatches:3


Location:chr15:45326884-45326907


Genomic Sequence:AGGAGGTGCTGAGAGCGAAGGGG


Strand:-


Genomic Region:gene(SORD)



3' PAM Orientation:AGGAGGTGCTGAGAGCGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||*||*||||||||||*||
AGGAGGTGCTGAGAGCGAAGGGG


Mismatch:

......T................
.........T.............
....................G..
Distal mismatch
Distal seed mismatch
PAM N Offtarget


13


Number of Mismatches:3


Location:chr5:16055461-16055484


Genomic Sequence:AGGAGGACGAGAGAGCGAAGTGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGACGAGAGAGCGAAGTGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||||||*||
AGGAGGACGAGAGAGCGAAGTGG


Mismatch:

.......C...............
........G..............
....................T..
Distal seed mismatch
Distal seed mismatch
PAM N Offtarget


14


Number of Mismatches:3


Location:chr3:19188578-19188601


Genomic Sequence:AGGTGGAGGAGAGAGGGAAGCGG


Strand:-


Genomic Region:intergenic



3' PAM Orientation:AGGTGGAGGAGAGAGGGAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||*||||*||||||*|||||||
AGGTGGAGGAGAGAGGGAAGCGG


Mismatch:

...T...................
........G..............
...............G.......
Distal mismatch
Distal seed mismatch
Proximal seed mismatch


15


Number of Mismatches:3


Location:chr12:132868776-132868799


Genomic Sequence:AGGTGCAGCAGAGAGCGTAGCGG


Strand:-


Genomic Region:gene(GALNT9)



3' PAM Orientation:AGGTGCAGCAGAGAGCGTAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||*|*|||||||||||*|||||
AGGTGCAGCAGAGAGCGTAGCGG


Mismatch:

...T...................
.....C.................
.................T.....
Distal mismatch
Distal mismatch
Proximal seed mismatch


16


Number of Mismatches:3


Location:chr5:122976928-122976951


Genomic Sequence:AGGAGGAAGAGAGAGCAAAGCGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAAGAGAGAGCAAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|||||||*||||||
AGGAGGAAGAGAGAGCAAAGCGG


Mismatch:

.......A...............
........G..............
................A......
Distal seed mismatch
Distal seed mismatch
Proximal seed mismatch


17


Number of Mismatches:3


Location:chr5:177988981-177989004


Genomic Sequence:AAGAGGCGCAGAGAGCGAAGCAG


Strand:+


Genomic Region:gene(COL23A1)



3' PAM Orientation:AAGAGGCGCAGAGAGCGAAGCAG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|*||||*||||||||||||||*|
AAGAGGCGCAGAGAGCGAAGCAG


Mismatch:

.A.....................
......C................
.....................A.
Distal mismatch
Distal mismatch
PAM mismatch


18


Number of Mismatches:3


Location:chr4:126512857-126512880


Genomic Sequence:AGGAGGAAGACAGAGCGAAGCGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAAGACAGAGCGAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||**|*||||||||||||
AGGAGGAAGACAGAGCGAAGCGG


Mismatch:

.......A...............
........G..............
..........C............
Distal seed mismatch
Distal seed mismatch
Proximal seed mismatch


19


Number of Mismatches:3


Location:chr7:104156642-104156665


Genomic Sequence:AGGAGAAGTACAGAGCGAAGCGG


Strand:+


Genomic Region:gene(LHFPL3)



3' PAM Orientation:AGGAGAAGTACAGAGCGAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||*||*|*||||||||||||
AGGAGAAGTACAGAGCGAAGCGG


Mismatch:

.....A.................
........T..............
..........C............
Distal mismatch
Distal seed mismatch
Proximal seed mismatch


20


Number of Mismatches:3


Location:chr9:976281-976304


Genomic Sequence:GGGAGGAGAAGAGAGAGAAGCGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:GGGAGGAGAAGAGAGAGAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
*|||||||*||||||*|||||||
GGGAGGAGAAGAGAGAGAAGCGG


Mismatch:

G......................
........A..............
...............A.......
Distal mismatch
Distal seed mismatch
Proximal seed mismatch


21


Number of Mismatches:3


Location:chr1:43471918-43471941


Genomic Sequence:AGGTGGAGCAGAGAGCCAAGGGG


Strand:-


Genomic Region:intergenic



3' PAM Orientation:AGGTGGAGCAGAGAGCCAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||*||||||||||||*|||*||
AGGTGGAGCAGAGAGCCAAGGGG


Mismatch:

...T...................
................C......
....................G..
Distal mismatch
Proximal seed mismatch
PAM N Offtarget


22


Number of Mismatches:3


Location:chr6:160390332-160390355


Genomic Sequence:AGCAGGAGCAGAGAGCGTTGCGG


Strand:-


Genomic Region:exon(NM_000876.exon0)



3' PAM Orientation:AGCAGGAGCAGAGAGCGTTGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||*||||||||||||||**||||
AGCAGGAGCAGAGAGCGTTGCGG


Mismatch:

..C....................
.................T.....
..................T....
Distal mismatch
Proximal seed mismatch
Proximal seed mismatch


23


Number of Mismatches:3


Location:chr13:77396114-77396137


Genomic Sequence:AGCAGGAGCAGAGAGGGAAGGGG


Strand:-


Genomic Region:intergenic



3' PAM Orientation:AGCAGGAGCAGAGAGGGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||*||||||||||||*||||*||
AGCAGGAGCAGAGAGGGAAGGGG


Mismatch:

..C....................
...............G.......
....................G..
Distal mismatch
Proximal seed mismatch
PAM N Offtarget


24


Number of Mismatches:3


Location:chr4:17424831-17424854


Genomic Sequence:AGGAGGAACAGAGAGCAAAGGGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAACAGAGAGCAAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||*||||||||*|||*||
AGGAGGAACAGAGAGCAAAGGGG


Mismatch:

.......A...............
................A......
....................G..
Distal seed mismatch
Proximal seed mismatch
PAM N Offtarget


25


Number of Mismatches:3


Location:chr6:35369238-35369261


Genomic Sequence:TGGAGGAGCAGAGAGCCAAGGGG


Strand:-


Genomic Region:gene(PPARD)



3' PAM Orientation:TGGAGGAGCAGAGAGCCAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
*|||||||||||||||*|||*||
TGGAGGAGCAGAGAGCCAAGGGG


Mismatch:

T......................
................C......
....................G..
Distal mismatch
Proximal seed mismatch
PAM N Offtarget


26


Number of Mismatches:3


Location:chr10:133184897-133184920


Genomic Sequence:GGGAGGAGCAGAGGGAGAAGCGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:GGGAGGAGCAGAGGGAGAAGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
*||||||||||||*|*|||||||
GGGAGGAGCAGAGGGAGAAGCGG


Mismatch:

G......................
.............G.........
...............A.......
Distal mismatch
Proximal seed mismatch
Proximal seed mismatch


27


Number of Mismatches:3


Location:chr3:14302029-14302052


Genomic Sequence:AGCAGGAGCAGAGAGAGAAGGGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGCAGGAGCAGAGAGAGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||*||||||||||||*||||*||
AGCAGGAGCAGAGAGAGAAGGGG


Mismatch:

..C....................
...............A.......
....................G..
Distal mismatch
Proximal seed mismatch
PAM N Offtarget


28


Number of Mismatches:3


Location:chrX:38284962-38284985


Genomic Sequence:AGGAGGAGTAGAGAGCTAAGAGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAGTAGAGAGCTAAGAGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*|||||||*|||*||
AGGAGGAGTAGAGAGCTAAGAGG


Mismatch:

........T..............
................T......
....................A..
Distal seed mismatch
Proximal seed mismatch
PAM N Offtarget


29


Number of Mismatches:3


Location:chr11:59965353-59965376


Genomic Sequence:AGGAGGAGGAGAGAGGGAAGGGG


Strand:-


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAGGAGAGAGGGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*||||||*||||*||
AGGAGGAGGAGAGAGGGAAGGGG


Mismatch:

........G..............
...............G.......
....................G..
Distal seed mismatch
Proximal seed mismatch
PAM N Offtarget


30


Number of Mismatches:3


Location:chr11:73715410-73715433


Genomic Sequence:AGCAGGAGCAGAGAGGGAAGCCG


Strand:-


Genomic Region:gene(UCP3)



3' PAM Orientation:AGCAGGAGCAGAGAGGGAAGCCG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||*||||||||||||*|||||*|
AGCAGGAGCAGAGAGGGAAGCCG


Mismatch:

..C....................
...............G.......
.....................C.
Distal mismatch
Proximal seed mismatch
PAM mismatch


31


Number of Mismatches:3


Location:chr10:93438057-93438080


Genomic Sequence:AGGGGGAGCAGAGAGAGAAGCAG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGGGGAGCAGAGAGAGAAGCAG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||*|||||||||||*|||||*|
AGGGGGAGCAGAGAGAGAAGCAG


Mismatch:

...G...................
...............A.......
.....................A.
Distal mismatch
Proximal seed mismatch
PAM mismatch


32


Number of Mismatches:3


Location:chr19:47572388-47572411


Genomic Sequence:AGGAGGAGGAGAGAGCGATGGGG


Strand:-


Genomic Region:exon(NM_015168.exon13)



3' PAM Orientation:AGGAGGAGGAGAGAGCGATGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*|||||||||*|*||
AGGAGGAGGAGAGAGCGATGGGG


Mismatch:

........G..............
..................T....
....................G..
Distal seed mismatch
Proximal seed mismatch
PAM N Offtarget


33


Number of Mismatches:3


Location:chr1:32928769-32928792


Genomic Sequence:AGGAGGAGGAGAGAGGGAAGGGG


Strand:-


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAGGAGAGAGGGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*||||||*||||*||
AGGAGGAGGAGAGAGGGAAGGGG


Mismatch:

........G..............
...............G.......
....................G..
Distal seed mismatch
Proximal seed mismatch
PAM N Offtarget


34


Number of Mismatches:3


Location:chr15:41164702-41164725


Genomic Sequence:AGGAGGAGGAGAGAGGGAAGGGG


Strand:-


Genomic Region:gene(RHOV)



3' PAM Orientation:AGGAGGAGGAGAGAGGGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*||||||*||||*||
AGGAGGAGGAGAGAGGGAAGGGG


Mismatch:

........G..............
...............G.......
....................G..
Distal seed mismatch
Proximal seed mismatch
PAM N Offtarget


35


Number of Mismatches:3


Location:chr10:22061873-22061896


Genomic Sequence:AGGAGGAACAGAGAGCGAGGGGG


Strand:+


Genomic Region:gene(DNAJC1)



3' PAM Orientation:AGGAGGAACAGAGAGCGAGGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||*||||||||||*|*||
AGGAGGAACAGAGAGCGAGGGGG


Mismatch:

.......A...............
..................G....
....................G..
Distal seed mismatch
Proximal seed mismatch
PAM N Offtarget


36


Number of Mismatches:3


Location:chr20:12613999-12614022


Genomic Sequence:AGGAGGAGGAGAGAGGGAAGGGG


Strand:-


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAGGAGAGAGGGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*||||||*||||*||
AGGAGGAGGAGAGAGGGAAGGGG


Mismatch:

........G..............
...............G.......
....................G..
Distal seed mismatch
Proximal seed mismatch
PAM N Offtarget


37


Number of Mismatches:3


Location:chr19:8018199-8018222


Genomic Sequence:AGGAGGAGGAGAGAGGGAAGGGG


Strand:-


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAGGAGAGAGGGAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||*||||||*||||*||
AGGAGGAGGAGAGAGGGAAGGGG


Mismatch:

........G..............
...............G.......
....................G..
Distal seed mismatch
Proximal seed mismatch
PAM N Offtarget


38


Number of Mismatches:3


Location:chr10:133184444-133184467


Genomic Sequence:AGGAGGAGCAGAGAGAGAGGCAG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAGCAGAGAGAGAGGCAG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||||||||||*||*||*|
AGGAGGAGCAGAGAGAGAGGCAG


Mismatch:

...............A.......
..................G....
.....................A.
Proximal seed mismatch
Proximal seed mismatch
PAM mismatch


39


Number of Mismatches:3


Location:chr1:158029953-158029976


Genomic Sequence:AGGAGGAGCAGAGAGGCAAGGGG


Strand:-


Genomic Region:gene(KIRREL)



3' PAM Orientation:AGGAGGAGCAGAGAGGCAAGGGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||||||||||**|||*||
AGGAGGAGCAGAGAGGCAAGGGG


Mismatch:

...............G.......
................C......
....................G..
Proximal seed mismatch
Proximal seed mismatch
PAM N Offtarget


40


Number of Mismatches:3


Location:chr11:24966728-24966751


Genomic Sequence:AGGAGGAGCAGAGACGGATGCGG


Strand:-


Genomic Region:gene(LUZP2)



3' PAM Orientation:AGGAGGAGCAGAGACGGATGCGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||||||||**||*||||
AGGAGGAGCAGAGACGGATGCGG


Mismatch:

..............C........
...............G.......
..................T....
Proximal seed mismatch
Proximal seed mismatch
Proximal seed mismatch


41


Number of Mismatches:3


Location:chr10:133183666-133183689


Genomic Sequence:AGGAGGAGCAGAGAGAAAAGCAG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAGCAGAGAGAAAAGCAG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
|||||||||||||||**||||*|
AGGAGGAGCAGAGAGAAAAGCAG


Mismatch:

...............A.......
................A......
.....................A.
Proximal seed mismatch
Proximal seed mismatch
PAM mismatch


42


Number of Mismatches:3


Location:chr16:90026480-90026503


Genomic Sequence:AGGAGGAGCAGAGAGCCAGGTGG


Strand:+


Genomic Region:gene(DEF8)



3' PAM Orientation:AGGAGGAGCAGAGAGCCAGGTGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||||||||||*|*|*||
AGGAGGAGCAGAGAGCCAGGTGG


Mismatch:

................C......
..................G....
....................T..
Proximal seed mismatch
Proximal seed mismatch
PAM N Offtarget


43


Number of Mismatches:3


Location:chr10:32430180-32430203


Genomic Sequence:AGGAGGAGCAGAGATCTAAGAGG


Strand:+


Genomic Region:intergenic



3' PAM Orientation:AGGAGGAGCAGAGATCTAAGAGG


Alignment:
AGGAGGAGCAGAGAGCGAAGCGG
||||||||||||||*|*|||*||
AGGAGGAGCAGAGATCTAAGAGG


Mismatch:

..............T........
................T......
....................A..
Proximal seed mismatch
Proximal seed mismatch
PAM N Offtarget











Paired Nickase
Cas9(D10A)



	
PairOffsetOverhang

		
hs0257132281650

		
hs0257132312559

		
hs0257132322660

		
hs0257132332963

		
hs0257132354175

		
hs0257132364276

		
hs0257132374579

		
hs0257132385084

		
hs0257132395185

		
hs0257132405589

		
hs0257132426397

		
hs02571324366100

		
hs02571324470104

		
hs02571324571105

		
hs02571324672106

		
hs02571324775109

		
hs02571324876110

		
hs02571325182116

		
hs02571325286120




Sequence



Surrounding genomic sequence (500bp upstream and downstream target site)


Note: Repeats and low complexity DNA is shown in lower case; non repeating sequence is shown in upper case.







	















Screening Analysis


Restriction EnzymeRecognition SiteMatchSequence


Psp10HIIGRAGCAGGGAGCAGAGGAGGAGCAGAGAGCGAAGCGG


RlaIVCWGCAAGGAGGAGCAGAGAGCGAAGCGG


Psp0357IIGCGAAGGCGAAGAGGAGGAGCAGAGAGCGAAGCGG


Cla11845IIIGCGAAGCGAAAGGAGGAGCAGAGAGCGAAGCGG




SNPs and Variation


Notes

	

        	×
        	Warning!  This gRNA contains a dinucleotide repeat sequence which can lead to greater off-targets.
    	




References



Jaskula-Ranga, V and Zack, DJ. 2016.  grID: A CRISPR-Cas9 guide RNA Database and Resource for Gene-Editing.
bioRxiv 097352; doi: https://doi.org/10.1101/097352


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