Database Information

S. pyogenes Cas9 sites Human Genome


Organism Genome Build Target Site Cas9 Occurrences grID prefix grID suffix
human hg19 N20NGG S. pyogenes (Spy) 137,409,562 hs
human hg19 N21NNGRRT S. aureus (Sa) 37,210,017 hs [sa]
human hg19 N18NNAGAAW S. thermophilus (St) 14,075,478 hs [st]
human hg19 N24NNNNGATT N. meningitidis (Nm) 10,219,504 hs [nm]

S. pyogenes Cas9 sites Mouse Genome


Organism Genome Build Target Site Cas9 Occurrences grID prefix grID suffix
mouse mm10 N20NGG S. pyogenes (Spy) 139,219,730 mm
mouse mm10 N21NNGRRT S. aureus (Sa) 38,584,448 mm [sa]
mouse mm10 N18NNAGAAW S. thermophilus (St) 13,517,569 mm [st]
mouse mm10 N24NNNNGATT N. meningitidis (Nm) 9,976,717 mm [nm]

S. pyogenes Cas9 sites for other genomes


Organism Genome Build Target Site Cas9 Occurrences grID prefix
human hg19 N20NGG S. pyogenes (Spy) 137,409,562 hs
human hg19 N20NAG S. pyogenes (Spy) 188,430,119 hs
mouse mm10 N20NGG S. pyogenes (Spy) 139,219,730 mm
rat rn5 N20NGG S. pyogenes (Spy) 143,165,607 rn
zebrafish danRer7 N20NGG S. pyogenes (Spy) 40,351,770 dr
C. elegans ce10 N20NGG S. pyogenes (Spy) 5,645,205 ce
S. cerevisiae sacCer3 N20NGG S. pyogenes (Spy) 944,171 sc

Engineered Cas9 Variants

High specificity S. pyogenes Cas9 variants


Organism Genome Build Target Site Cas9 Occurrences grID prefix grID suffix
human hg19 N20NGG SpCas9(D1135E) 137,409,562 hs
human hg19 N20NGG eSpCas9 137,409,562 hs
human hg19 N20NGG SpCas9-HF1 137,409,562 hs

Alternative PAM variants

Organism Genome Build Target Site Cas9 Occurrences grID prefix grID suffix
human hg19 N20NGAN SpCas9 (VQR) 160,191,547 hs [NGAN]
human hg19 N20NGAG SpCas9 (EQR) 42,727,086 hs [NGAG]
human hg19 N20NGCG SpCas9 (VRER) 5,968,752 hs [NGCG]

Alternative CRISPR systems (CRISPR-Cpf1)


Organism Genome Build Target Site CRISPR System Occurrences grID prefix grID suffix
human hg19 TTTNN23 AsCpf1 or LbCpf1 99,828,092 hs [Cpf1]

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